EFFECT OF NUTRITIONAL MEDIA, SUCROSE AND THIDIAZURON ON THE ABILITY OF SOMATIC EMBRYO REGENERATION THROUGH PLUMULE CULTURE OF DWARF GREEN COCONUT

Abstract

Coconut is an important economic crop, especially green coconut which is widely grown in the Mekong river delta with increasing demand for fresh coconut water. This study focuses on the application of somatic embryo culture technique to propagate quality green coconut, addressing the urgent need today. Three factors were investigated: the effects of shoot culture time and sugar concentration on embryogenic callus formation, and the effects of growth regulator TDZ on embryogenic callus regeneration. The results showed that 14-day embryo culture and the use of 45 g/L sugar resulted in the highest embryogenic callus formation efficiency. During regeneration, TDZ concentrations of 25 µM and 50 µM showed better regeneration ability than other concentrations. The study has initially created and regenerated embryogenic callus effectively for Vietnamese green coconut, opening up a potential direction for coconut breeding research.