APPLICATION OF MULTIPLEX RT-PCR FOR DETECTION OF Cymbidium mosaic virus (CymMV) AND Odontoglossum ringspot virus (ORSV) CAUSING DISEASES IN THE GENUS Dendrobium ORCHIDS

Abstract

Dendrobium orchids are beautiful and precious cut flowers with high economic potential. However, virus diseases in orchids is a major barrier causing very negative impact on the orchid growing industry. The purpose of this study was to apply multiplex RT-PCR to detect and survey the infection rate of CymMV and ORSV in the orchid genus Dendrobium. The study results demonstrated that the virus detection capacity from the multiplex RT-PCR completely identical to the virus detection results from the simple RT-PCR. Thus, the results showed that the multiplex RT-PCR applied in this study was a rapid, simple, accurate and reliable technique. In addition, the results of using multiplex RT-PCR on 40 Dendrobium orchid samples collected from the orchid garden detected 26 CymMV infections, 2 ORSV infections, two of which 2 ORSV infections. Two of which were co-infected with CymMV and ORSV. The archived results of this study were useful information contributing to virus research on the genus Dendrobium orchid in Vietnam.