MICROPROPAGATION OF THE KOREAN CHRYSANTHEMUM (Chrysanthemum morifolium) AT CHAU THANH, AN GIANG

Abstract

The Korean chrysanthemum (Chrysanthemum morifolium) belongs to the Asteraceae family. In Vietnam, this species has been introduced and favoured for its vibrant colours, providing high economic benefits. Although general micropropagation techniques exist for Chrysanthemum species, a specialized protocol tailored to Korean chrysanthemum has yet to be developed. This study aimed to investigate the effects of BAP and NAA on shoot and root formation in vitro on the shoots of Chrysanthemum morifolium and to determine the optimal substrate ratio for acclimatisation ex vitro. The results showed that MS medium supplemented with 0.5 mg/L BAP was the best for shoot formation (20.11 shoots per explant, with an average height of 4.89 cm and an average of 1.13 leaves per shoot). The MS medium supplemented with NAA 1.5 mg/L was the best for root number formation (20.44 roots per shoot, average root length of 2.75 cm, 17.22 leaves per shoot). The suitable substrate ratio for acclimatising the Korean chrysanthemum ex vitro was 30% Tribat soil, 60% coconut fibre, and 10% sand, achieving a survival rate of 100%, an average plant height of 6.43 cm, and 22.17 leaves per sample.