IN VITRO PROPAGATION OF BA BE CYMBIDIUM ORCHID (Cymbidium aloifolium (L.) Sw.)

Abstract

This study aims to develop an in vitro propagation protocol for the Ba Be Cymbidium orchid (Cymbidium aloifolium (L.) Sw.) to contribute to the conservation of this species, provide a basis for related research and supply orchid seedlings to the market. Orchid capsules were cleaned and sterilized with 0.1% HgCl₂ solution for 20 minutes, resulting in a contamination - free survival rate of 66.67%. The optimal basal medium for propagation was MS + 30 g/L sucrose + 5.5 g/L agar, pH 5.6 - 5.8, achieving a shoot multiplication coefficient of 1.69. To enhance the efficiency of shoot multiplication, the basal medium was supplemented with the following: Coconut water (100 ml/L), resulting in a shoot multiplication coefficient of 3.37; BAP (1 mg/L), achieving a coefficient of 3.76; kinetin (1.5 mg/L), yielding a coefficient of 3.52. During the rooting stage, culture samples grown on a basal medium supplemented with 1.5 mg/L NAA achieved a root multiplication coefficient of 3.72. The addition of 1 g/L activated charcoal (AC) to the medium containing 1.5 mg/L NAA further optimized the root multiplication coefficient, reaching 4.4. The best substrate for acclimatizing in vitro seedlings was a mixture of wood and pumice (1: 1), achieving a survival rate of 93.33%.