OPTIMIZATION OF STOCKING DENSITY AND HOLDING TEMPERATURE FOR LIVE STORAGE OF COMMERCIAL CUTTLEFISH (Sepia spp.) USING RESPONSE SURFACE METHODOLOGY

Abstract

The study aimed to determine the optimal stocking density and holding temperature for live storage of commercial cuttlefish (Sepia spp.), thereby enhancing preservation efficiency and economic value. The experiment was conducted in two stages (pilot and practical scale) with stocking densities of 10, 15, 20 kg/500 L and temperatures of 21, 24, 27°C. Response Surface Methodology (RSM) was applied to analyze the effects and identify optimal values. Results showed that both density and temperature significantly affected survival rates (p < 0.05), with density having a stronger influence. The highest survival rate (100%) was achieved at 10 kg/500 L and 21 or 24°C, while high density and high temperature (20 kg/500 L; 27°C) together reduced survival to 71.4 - 78.6%. The second-order regression model identified optimal density and temperature at 17.9 kg/500 L and 22.8°C, respectively, which maintained survival rates above 85% during storage. These results provide the basis for developing a live storage technology process for cuttlefish, applicable in practical production at facilities engaged in the trade and consumption of live cuttlefish.