STUDY OF PRODUCTION TECHNOLOGY OF POLYSACCHARIDES-RICH EXTRACT FROM Ganoderma lucidum BY ULTRASONIC-ASSISTED ENZYMATIC METHOD ON A PILOT SCALE

Abstract

Based on the results of laboratory-scale research, we selected the technological parameters for producing polysaccharides-rich extract powder from Ganoderma lucidum on a scale of 300 liters per batch. G. lucidum is crushed to a size of 1 - 2 cm x 0.5 - 1 cm, then mixed with a ratio of mushroom material/solvent of 1 : 20, added with 0.5% celluclast enzym. The suitable extraction conditions were carried out by ultrasonication at a power of 600 W for 35 minutes at 50^oC, then continuing in incubation and extraction at 50^oC for 115 minutes. The polysaccharides-rich extract solution was concentrated by using vacuum evaporation until the dissolved solids content reached 1.6°Brix, under the pressure of 700 mmHg, concentration temperature of 60°C and the estimated time of 4.5 hours. The extract powder was produced using the freeze-drying method, adding maltodextrin as the carrier at a ratio of 3%, and the material thickness of 1 cm. The raw material was frozen at -30°C for 6 hours and freeze-dried under a pressure of 30 - 60 Pa, with a condenser temperature of -60°C, for 40 hours. The dry extract powder had a moisture content of 4.5%, meeting the quality requirements for dry extract powder as per the Vietnamese Pharmacopoeia V (<5%). The polysaccharides content was 4.105 g/100 g (4.1%) and the food safety criteria complied with the regulations in the Vietnamese Pharmacopoeia V and Decision 46/2007/QD-BYT on 19^th December, 2007, by the Ministry of Health in Vietnam. The product was a loose, dry powder with a gray-brown color and the characteristic fragrance of G. lucidum.