OPTIMIZATION OF CULTURE MEDIUM INGREDIENTS TO STIMULATE SPORULATION OF THE ANTAGONISTIC Bacillus pumilus TG-71 AGAINST RICE BACTERIAL LEAF BLIGHT (Xanthomonas oryzae pv. oryzae)
DOI:
https://doi.org/10.71254/wxtwax27Keywords:
Bacillus pumilus, central composite designs, Plackett-Burman matrix, response surface methodology, sporulation.Abstract
The antagonistic Bacillus pumilus TG-71 against rice bacterial leaf blight was isolated from a rice field in Tien Giang Province of Vietnam. Using the Plackett-Burman matrix, three culture medium ingredients including peptone, (NH4)2SO4 and CaCl2.2H2O were found to be major factors having impacts on TG-71 sporulation. Values of these three factors were optimized using the response surface methodology (RSM) combined with the central composite designs (CCD). Optimal values of peptone, (NH4)2SO4 and CaCl2.2H2O were 12 g/l, 4 g/l and 2.699 g/l, respectively. Spore density was 9.4 x 106 CFU/ml using these values.
