ISOLATION AND EVALUATION OF ANTIBACTERIAL ACTIVITY OF ACTINOMYCES ISOLATES ON Fusarium oxysporum CAUSING YELLOW WILT DISEASE ON VEGETABLE SOYBEAN

Abstract

This study aimed to examine the antagonistic ability of actinomycetes against Fusarium oxysporum, which causes yellow wilt disease on soybean. Among 26 isolates tested, four isolates (PT-AG9, CM-AG27, PT-AG16, CM-AG33) showed strong inhibition with inhibition zones ranging from 13.50 mm to 14.25 mm and antagonistic efficacy from 68.33% to 76.39% after 9 days. These isolates also produced β-glucanase and chitinase enzymes. PT-AG16 had the highest β-glucanase activity with a lysis halo of 12.88 mm at 14 days, while PT-AG16 and CM-AG33 showed the strongest chitinolytic activity with halo diameters of 26.50 mm and 26.25 mm at 7 days.