IDENTIFICATION OF THE AGENT CAUSING ANTHRACNOSE DISEASE ON MANGO FRUIT IN BA RIA - VUNG TAU PROVINCE AND EFFICACY OF CHITOSAN AND CHITOSAN NANOPARTICLES AGAINST THE PATHOGEN

Abstract

Colletotrichum spp. causing anthracnose disease are responsible for approximately 30 - 60% of global mango yield losses. This study aimed to identify the pathogen of anthracnose on mangoes in Ba Ria – Vung Tau province and to evaluate the efficacy of chitosan and chitosan nanoparticles against Colletotrichum gloeosporioides under laboratory conditions. The research contents consisted of species identification using molecular biology techniques and assessment of the antifungal activity of chitosan and chitosan nanoparticles through the medium toxicity method. The results confirmed C. gloeosporioides as the pathogen causing anthracnose symptom on Hoa Loc mango fruits in Ba Ria - Vung Tau province. Both chitosan and chitosan nanoparticles with a concentration of 1000 ppm completely inhibited spore germination within 6 hours of infection. Furthermore, at a concentration of 2500 ppm, chitosan and chitosan nanoparticles prevented 49.51% and 100% of mycelial growth on PDA medium at 5 days after culture, respectively. The minimum inhibitory concentration required to achieve 90% inhibition (MIC₉₀) of spore germination was 799 ppm for chitosan and 755 ppm for chitosan nanoparticles. Regarding mycelial growth inhibition, the MIC₉₀ values for chitosan and chitosan nanoparticles, respectively, were 4267 ppm and 2407 ppm after 5 days of culture.