STUDYING ON THE HEAT AND MICROWAVE ASSISTED EXTRACTION OF PHENOLIC COMPOUNDS FROM MING ARALIA (Polyscias fruticosa L.) LEAVES

Abstract

Polyscias fruticosa is a medicinal plant widely used for medicine due to its antioxidant, anti-inflammatory and antipyretic activities. Two main factors affecting the extraction process are temperature (30 to 90^oC, the interval temperature for each treatment is 10^oC) and extraction time (10 to 60 minutes, the interval time for each treatment is 10 minutes) were a factorial design in this experiment. Microwave-assisted extraction (MAE) was a two-factor experiment: microwave power (70, 210 and 350 W) and extraction time (10 to 30 seconds with interval time for each treatment being 5 seconds). The extraction proceeds with a ratio of raw material/water being 1/25 (w/v). The efficiency of phenolic compounds extraction in Polyscias fruticosa leaves expressed through the total phenolic content (TPC), total flavonoid content (TFC) and the DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging in the extracted material. The results of multiple-regression analysis of TPC, TFC and the DPPH free radical scavenging according to temperature and extraction time and the results of solving the differential equation to find the optimal parameters show that extraction at 60.0^oC for 39.7 minutes TPC reach the maximum (29.1 mg GAE/g), a temperature of 63.5^oC with a time of 42.8 minutes for optimal TFC (15.1 mg QE/g) and a temperature of 55.7^oC with a time of 49.2 minutes for optimal free radical elimination ability (69.0%). The microwave-assisted extraction method is more effective. At a power of 210 W and 25 seconds, TPC, TFC and DPPH free radical scavenging were 35.1 mg GAE/g, 22.0 mg QE/g and 79.6%, respectively.