EFFECTS OF CULTURE CONDITIONS ON MYCELIAL GROWTH AND FRUIT BODY OF LINGZHI MUSHROOM Ganoderma orbiforme (Fr.) Ryvarden

Abstract

Lingzhi mushrooms (Ganoderma spp.) are well-known for their numerous health benefits in disease prevention and treatment. These benefits come from their rich content of biologically active compounds. However, there are limited studies on the Ganoderma orbiforme species in Vietnam. This study aimed to identify the optimal growth culture conditions of G. orbiforme strain Go1, which was collected from Xuan Son National Park, Phu Tho province, Vietnam. The results showed that the mycelial of strain Go1 grew well in a PDA medium at pH 6.0 - 8.0 and at a temperature of 25 - 30^oC. Rice, millet, and wheat can be used as substrates for producing the mother spawn of strain Go1. Among these, rice and wheat promoting the fastest mycelial growth. Strain Go1 was able to form fruiting bodies when cultivated on substrates, including rubber sawdust and corn cobs. The substrate composition significantly affected the biological performance of strain Go1. The optimal formula consisted of 89% sawdust, 10% rice bran, and 1% calcium carbonate. Using this formula biological efficiency reached 8.94 ± 0.23%.